Anti-Calreticulin Antibody

Rabbit Polyclonal Antibody specific to Calreticulin Antibody

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

CRP55, Calregulin, Endoplasmic reticulum resident protein 60, ERp60, HACBP, grp60

Gene Name

CALR

NCBI Gene ID

UniProt

P27797

Cellular Locus

Endoplasmic reticulum lumen, Cytoplasm, cytosol, Secreted, extracellular space, extracellular matrix, Cell surface, Sarcoplasmic reticulum lumen, Cytoplasmic vesicle, secretory vesicle, Cortical granule, Cytolytic granule

Host

Rabbit

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea Pig, Monkey, Pig, Hamster, Rabbit, Sheep

Immunogen

Human calreticulin synthetic peptide conjugated to KLH.

Target Antigen

Calreticulin

Target

Calreticulin

Clonality

Polyclonal

Type

Antibody

Applications

Field of Research

Neuroscience

Purification Method

Whole antiserum

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Whole Antiserum

Form

Liquid

Buffer

Whole Antiserum

Function

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed:7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed:11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity). {UniProtKB:P28491, UniProtKB:Q8K3H7, PubMed:11149926, PubMed:7876246}.

Additionnal Information

Immunoblotting: use at 1:5,000-1:10,000 dilution. A band of ~63 kDa is detected. <br><br>These are recommended concentrations. User should determine optimal concentrations for their application. <br><br>Positive control: HeLa cell lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, rat, bovine, canine, chicken, guinea pig, monkey, pig, hamster, rabbit, and sheep calreticulin.

Formulation

Whole antiserum

Target Background

Calreticulin is a multifunctional highly conserved Ca+2-binding protein that is localized primarily in the endoplasmic reticulum (ER) but has also been detected in the nucleus and nuclear envelope. Like many other ER proteins, it has the conserved ER retention KDEL sequence (Lys-Asp-Glu-Leu) at its C-terminus. Calreticulin is made up of three domains: 1 180 residue N-terminal domain, a proline- rich P-domain (aa 189-288) that binds Ca+2 with high affinity and shares homology with calnexin and calmegin, and a 110 residue C-terminal domain that binds Ca+2 with low affinity but high capacity. Calreticulin appears to be involved in calcium storage and regulation, has molecular chaperone activity, and has been shown to be induced in response to various types of cell stress.