Recombinant Mouse CCL2
Product Specifications
Product Name Alternative
AI323594, Ccl2, chemokine (C C motif) ligand 2, HC11, JE, MCAF, MCP 1, Mcp1, Scya2, Sigje, SMC CF
UniProt
P10148
Expression Region
24-96
Host
E.Coli
Tag
N-terminal His Tag
Applications
Western Blot, ELISA
Endotoxin
< 1 EU/µg as determined by LAL test.
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR2A. The ED50 for this effect is typically 5-15 ng/mL.
Form
Lyophilized powder
Reconstitution
Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.
Molecular Weight
8.5 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.
Formulation
Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.
Organism Species
Mouse
Available Sizes
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