Anti-Atg13 Antibody

Rabbit Polyclonal Antibody specific to Atg13

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Atg13 Rabbit Polyclonal Antibody

Gene Name

ATG13

NCBI Gene ID

UniProt

O75143

Cellular Locus

Cytoplasm, cytosol, Preautophagosomal structure

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to mid-protein amino acids of human Atg13.

Target Antigen

Autophagy-related protein 13

Target

Atg13

Clonality

Polyclonal

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Autophagy

Purification Method

Purified by Protein A affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation. {PubMed:18936157, PubMed:19211835, PubMed:19225151, PubMed:19287211, PubMed:21795849, PubMed:21855797}.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. A band of ~56-63kDa is detected. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human Atg13.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Concentration: 1mg/ml

Buffer pH

pH 7.4

Target Background

Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. Atg13 is a target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of Atg13 and ULK1, and the regulation of the Atg13-ULK1-RB1CC1 complex. Under starvation conditions, Atg13 is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation. Phosphorylation of Atg13 depends on nutrient conditions; it is dephosphorylated during starvation or following treatment with rapamycin. Three isoforms of the human protein are produced by alternative splicing.