Anti-DNA Polymerase δ Antibody

Mouse Monoclonal Antibody specific to DNA Polymerase δ

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 2.7.7.7, 3'-5' exodeoxyribonuclease, EC 3.1.11.-, DNA polymerase subunit δ p125

Gene Name

POLD1

NCBI Gene ID

UniProt

P28340

Accession Number

NP_001243778.1

Cellular Locus

Nucleus

Host

Mouse

Reactivity

Human

Immunogen

Recombinant protein corresponding to aa 1-140 of the large subunit of human DNA polymerase delta

Target Antigen

DNA polymerase δ catalytic subunit

Target

DNA Polymerase δ

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC-P

Field of Research

Enzymes

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24035200, PubMed:24022480). Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine, 8oxoG or abasic sites (PubMed:19074196, PubMed:24191025). {PubMed:16510448, PubMed:19074196, PubMed:20227374, PubMed:20334433, PubMed:24022480, PubMed:24035200, PubMed:24191025, PubMed:24310611}.

Additionnal Information

Immunoblotting: use at a 1:500-1:3,000 dilution. A band of ~124kDa is detected. Detection of DNA polymerase delta in HeLa cell lysate with #13910 diluted 1:1,000 <br><br>IHC: use at a dilution of 1:200 with antigen retrieval: citrate buffer, pH 6.0, 15 minutes. Detection of DNA polymerase delta in paraffin-embedded human colon carcinoma. <br><br>Endusers should determine optimal antibody concentrations for their applications.