1-Benzyl-I3C

1-Benzyl-I3C is a NEDD4-1 inhibitor with significant anticancer activity. 1-Benzyl-I3C can directly inhibit the ubiquitination activity of NEDD4-1 with an IC50 of 12.3μM, which is significantly better than its precursor compound I3C of 284μM. 1-Benzyl-I3C and its analogs showed good effects in inhibiting the proliferation of human melanoma cells, which is roughly related to their potency as NEDD4-1 enzyme inhibitors. By combining in vitro ubiquitination experiments and thermal stability analysis, 1-Benzyl-I3C was shown to be able to bind to the catalytic HECT domain of NEDD4-1[1].

Product Specifications

CAS Number

[60941-76-6]

UNSPSC

12352005

Hazard Statement

H315, H319, H320

Target

E1/E2/E3 Enzyme

Type

Natural Products

Related Pathways

Metabolic Enzyme/Protease

Applications

Cancer-programmed cell death

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/1-benzyl-i3c.html

Purity

99.68

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

OCC1=CN(CC2=CC=CC=C2)C3=C1C=CC=C3

Molecular Formula

C16H15NO

Molecular Weight

237.30

Precautions

H315, H319, H320

References & Citations

[1]Indole-3-carbinol (I3C) analogues are potent small molecule inhibitors of NEDD4-1 ubiquitin ligase activity that disrupt proliferation of human melanoma cells