PACT (PKR activating protein) / PRKRA Recombinant Rabbit Monoclonal Antibody [PSH02-15]
PRKRA can activate EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
PRKRA, PACT, RAX, HSD-14, HSD14
Swiss Prot
O75569 Human, Q9WTX2 Mouse, Q4V8C7 Rat
Cellular Locus
Cytoplasm, perinuclear region
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Recombinant protein within human PACT aa 1-313 (O75569).
Isotype
IgG
Clone
PSH02-15
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IHC-P, IF-Cell, IF-Tissue, FC
Positive Control
Concentration
1 mg/mL
Dilution
WB: 1:1,000 ;IHC-P: 1:1,000-1:5,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:200 ;FC: 1 μg/mL
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 34 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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