Anti-DRAK2 (CT) Antibody

Rabbit Polyclonal Antibody specific to DRAK2 (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 2.7.11.1, DAP kinase-related apoptosis-inducing protein kinase 2

Gene Name

STK17B

Gene ID

9262

NCBI Gene ID

UniProt

O94768

Accession Number

NP_004217.1

Cellular Locus

Nucleus, Cell membrane, Endoplasmic reticulum-Golgi intermediate compartment

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 351-365 of human DRAK2 (accession no. AB011421).

Target Antigen

Serine/threonine-protein kinase 17B

Target

DRAK2 (CT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Phosphorylates myosin light chains (By similarity). Acts as a positive regulator of apoptosis. {PubMed:9786912}.

Additionnal Information

Immunoblotting: use at 1ug/mL. Immunocytochemistry: use at 10ug/mL. Positive control: Whole cell lysate from Jurkat or Raji cells. These are recommended concentrations. Enduser should determine optimal concentrations or their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human DRAK2 (45kDa). No cross-reactivity with DRAK1, DAP or ZIP kinases.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis have been identified and designated DRAK1 and DRAK2 for DAP kinaseâ€“related apoptosis-inducing protein kinases. DRAKs contain an N-terminal kinase domain and a C- terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases which mediate apoptosis through their catalytic activities. DRAK2 is located in cell nuclei, and the mRNA for DRAK2 is ubiquitously expessed in human tissues.