eIF3g Recombinant Rabbit Monoclonal Antibody [PSH09-42]
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
Eukaryotic translation initiation factor 3 subunit G, eIF3g, EIF3G, EIF3S4
Swiss Prot
O75821 Human, Q9Z1D1 Mouse, Q5RK09 Rat
Cellular Locus
Cytoplasm, Nucleus, perinuclear region.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Recombinant protein within human eIF3g aa 1-320.
Isotype
IgG
Clone
PSH09-42
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB
Positive Control
K-562 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, RAW264.7 cell lysate, PC-12 cell lysate.
Concentration
1 mg/mL
Dilution
WB: 1:2,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 36 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
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