Unconjugated Human HLA-A*24:02&B2M&EBV BRLF1 (TYPVLEEMF) Complex Protein (Monomer, MALS verified)

Unconjugated Human HLA-A*24:02&B2M&EBV BRLF1 (TYPVLEEMF) Complex Protein (HLF-H52E5) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Thr 305 (HLA-A*24:02) & Ile 21 - Met 119 (B2M) & TYPVLEEMF peptide (Accession # AAA59600.1 (HLA-A*24:02) & P61769 (B2M) & TYPVLEEMF) .

Product Specifications

Specifications

Unconjugated Human HLA-A*24:02&B2M&EBV BRLF1 (TYPVLEEMF) Complex Protein is produced by co-expression of HLA and B2M loaded with EBV BRLF1 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™), and it is not biotinylated. The protein has a calculated MW of 35.8 kDa and 11.7 kDa. The protein migrates as 39-42 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAA59600.1 (HLA-A*24:02) & P61769 (B2M) & TYPVLEEMF

Expression Region

Gly 25 - Thr 305 (HLA-A*24:02) & Ile 21 - Met 119 (B2M) & TYPVLEEMF peptide

Host

HEK293

Target

HLA-A*2402 & B2M & EBV BRLF1 (TYPVLEEMF)

Conjugation

Unconjugated

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Anti-HLA class I Antibody, Human IgG1 (W6/32) at 1 μg/mL (100 μL/well) can bind Unconjugated Human HLA-A*24:02&B2M&EBV BRLF1 (TYPVLEEMF) Complex Protein (Cat. No. HLF-H52E5) with a linear range of 5-40 ng/mL (QC tested).