Cytokeratin 5/8 (C-50), CF740 conjugate, 0.1mg/mL
This antibody reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa) . Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Specifications
CAS Number
9007-83-4
Synonyms
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
KRT5
Gene ID
3852 (CK5); 3856 (CK8)
NCBI Gene ID
433845 (CK5); 533782; 708445 (CK8)
UniProt
P13647 (CK5); P05787 (CK8)
Swiss Prot
P13647 (CK5) ; P05787 (CK8)
Host
Mouse
Species Reactivity
Cow,Dog,Ferret,Hamster,Human,Mouse,Pig,Rat,Sheep
Reactivity
Cytokeratin 5/8
Immunogen
Cytoskeletal preparation from HeLa cells
Target Antigen
Cytokeratin 5/8
Clonality
Monoclonal
Clone
C-50
Type
Primary
Source
Animal
Origin
Animal
Applications
IHC, FFPE (verified)
Field of Research
Cancer, Cytoskeleton
Positive Control
HepG2 or A431 cells. Skin or Squamous cell carcinoma
Stability
2 years
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% rBSA, 0.05% azide
Molecular Weight
58 kDa (CK5) ; 52.5 kDa (CK8)
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Notes
Protect from light
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG1, kappa
Species
Mus musculus (mouse), Bos taurus (bovine)
Classification
0.05% sodium azide (CAS 26628-22-8)
Conjugate
CF740
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