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Anti-Biotin(Hyb8)

This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

Product Specifications

Synonyms

Vitamin B7 or Vitamin H

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

Not Applicable

UniProt

Not Applicable

Host

Mouse

Species Reactivity

Pan species

Immunogen

Biotinylated sheep immunoglobulin

Target Antigen

Biotin

Clonality

Monoclonal

Isotype

IgG1

Clone

Hyb8

Conjugation

Purified, BSA-free

Source

Animal

Applications

IHC, FFPE (verified)

Validated Applications

IHC,FFPE

Positive Control

Biotinylated proteins in solution or on tissues

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

244Da

Additionnal Information

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|No special pretreatment is required for the immunohistochemical staining of formalin-fixed paraffin-embedded tissues.|Immunohistology formalin-fixed 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting

Shipping Conditions

Room temperature

Storage Conditions

-35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

2 years

Product Datasheet

https://cdn.gentaur.com/products/37/865540/datasheet/bnum0400-50.pdf

Product MSDS

https://cdn.gentaur.com/products/37/865540/msds/bnum0400-50.pdf

CAS Number

9007-83-4

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