Goat anti-RFP Polyclonal antibody

Goat polyclonal antibody to RFP (Red fluorescent protein) conjugated to DyLight® 650. RFP is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma.

Product Specifications

CAS Number

9007-83-4

Specifications

In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).

Product Name Alternative

Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.

UNSPSC Description

Red Fluorescent Protein

Volume

100 µL

Host

Goat

Reactivity

Red Fluorescent Protein (dsRed), mCherry, mOrange, mPlum, mRFP, mStrawberry and tdTomato.

Immunogen

Purified recombinant RFP peptide produced in E. coli.

Target Antigen

Purified recombinant RFP peptide produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-RFP, DyLight®650

Clonality

Polyclonal

Isotype

IgG

Conjugation

DyLight®650

Type

Primary

Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK

Applications

WB, IF, IHC-F

Purification

Epitope affinity purified

Concentration

2.5 mg/mL

Dilution

WB:1:500-1:5,000, IF:1:50-1:500, IHC-F:1:50-1:1,000

Form

Polyclonal antibody supplied as a 100 µl (2.5 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

Storage Conditions

For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.