What is the catalog number for Hoechst 33258?

The catalog number for Hoechst 33258 is HY-15558.

What sizes are available for Hoechst 33258?

Hoechst 33258 is available in the following size(s): 50 mg, 100 mg.

How many pack sizes does Hoechst 33258 come in?

Hoechst 33258 is available in 2 different pack sizes.

Is Hoechst 33258 in stock?

Hoechst 33258 is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Hoechst 33258?

Hoechst 33258 is manufactured by QuantiMol.

What is the estimated delivery time for Hoechst 33258?

Estimated delivery time for Hoechst 33258 is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Hoechst 33258?

The safety data sheet for Hoechst 33258 is available here: http://file.medchemexpress.com/batch_PDF/HY-15558/Hoechst-33258-SDS-MedChemExpress.pdf.

Hoechst 33258

Hoechst 33258 is a blue to blue-green fluorescent live cell dye that can label DNA. Hoechst 33258 can specifically bind to the minor groove of DNA (and tends to bind to A/T-rich DNA), resulting in a significant increase in fluorescence intensity. Hoechst 33258 can cross the cell membrane and cause changes in DNA structure, such as G2/M phase arrest. Hoechst 33258 can bind to live or fixed cells, and the fluorescence intensity increases with increasing solution pH. As a DNA-specific probe, Hoechst 33258 can be used to detect DNA content, analyze cell cycle, etc. The excitation wavelength of Hoechst 33258 is 350-365 nm, and the emission wavelength is 460-490 nm[1][2][3].

Product Specifications

CAS Number

23491-44-3

Product Name Alternative

BisBenzimide H 33258; H 33258

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

DNA Alkylator/Crosslinker; DNA Stain; Fluorescent Dye

Type

Dye Reagents

Related Pathways

Cell Cycle/DNA Damage; Others

Applications

Cancer-programmed cell death

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/Hoechst-33258.html

Purity

99.93

Solubility

DMSO : 50 mg/mL (ultrasonic)

Smiles

OC1=CC=C(C2=NC3=CC=C(C4=NC5=CC=C(N6CCN(C)CC6)C=C5N4)C=C3N2)C=C1

Molecular Formula

C25H24N6O

Molecular Weight

424.50

Precautions

H302, H315, H319, H335

References & Citations

[1]Stokke T, et al. Multiple binding modes for Hoechst 33258 to DNA. J Histochem Cytochem. 1985 Apr;33 (4) :333-8.|[2]Wang XJ, et al. Newly synthesized bis-benzimidazole derivatives exerting anti-tumor activity through induction of apoptosis and autophagy. Bioorg Med Chem Lett. 2012 Oct 1;22 (19) :6297-300.|[3]Pjura PE, et al. Binding of Hoechst 33258 to the minor groove of B-DNA. J Mol Biol. 1987 Sep 20;197 (2) :257-71.