Anti-NALCN Antibody

Mouse Monoclonal Antibody specific to NALCN

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Four domain-type voltage-gated ion channel α-1 subunit, Rb21-channel, Sodium leak channel non-selective protein, Voltage gated channel-like protein 1

Gene Name

Nalcn

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=Nalcn">Nalcn</a>

UniProt

Q6Q760

Accession Number

NP_705894.1

Cellular Locus

Cell membrane, Multi-pass membrane protein

Host

Mouse

Reactivity

Mouse, Rat

Immunogen

Fusion protein corresponding to aa 1659-1738 (cytoplasmic C- terminus) of rat NALCN (accession no.Q6Q760).

Target Antigen

Sodium leak channel NALCN

Target

NALCN

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Voltage-gated ion channel responsible for the resting Na(+) permeability that controls neuronal excitability. NALCN channel functions as a multi-protein complex, which consists at least of NALCN, NALF1, UNC79 and UNC80. NALCN is the voltage-sensing, pore-forming subunit of the NALCN channel complex. NALCN channel complex is constitutively active and conducts monovalent cations but is blocked by physiological concentrations of extracellular divalent cations (By similarity). In addition to its role in regulating neuronal excitability, is required for normal respiratory rhythm, systemic osmoregulation by controlling the serum sodium concentration and in the regulation of the intestinal pace-making activity in the interstitial cells of Cajal. NALCN channel is also activated by neuropeptides such as neurotensin and substance P (SP) through a SRC family kinases-dependent pathway. In addition, NALCN activity is enhanced/modulated by several GPCRs, such as CHRM3 (By similarity). {UniProtKB:Q8BXR5, UniProtKB:Q8IZF0}.

Additionnal Information

Immunoblotting: use at 1ug/mL. Predicted molecular weight is ~200kDa. Positive control: rat brain lysate. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes mouse and rat NALCN.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

NALCN (sodium leak channel non- selective protein) is a 1,738 amino acid multi-pass membrane protein in the cation-nonselective channel family. NALCN is highly conserved in mammalian species and is widely expressed in the central nervous system. NALCN is a voltage-independent, non-selective cation channel which is permeable to sodium, potassium, and calcium ions. It is responsible for background sodium ion leak conductance in neurons and regulates basal excitability of the nervous system. Three isoforms of NALCN result from alternative splicing events.