Anti-DOG1 Antibody

Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, Transmembrane protein 16A, Tumor-amplified and overexpressed sequence 2

Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. {PubMed:20056604, PubMed:21984732, PubMed:22178883, PubMed:22946059}.

<br><br>Immunohistochemistry: Use at 1- 10ug/ml on paraffin-embedded samples. Antigen retrieval solution: 10mM citrate, pH 6 (boil slides by microwaving in antigen retrieval solution for 12 minutes).User should determine optimal concentrations for their applications.

DOG1 (Discovered on GIST1), also known as TMEM16A and ANO1, has emerged in recent years as a promising biomarker for gastrointestinal stromal tumors (GIST). It was originally discovered through microarray expression profiling analysis as a gene that is highly expressed in GIST, and subsequent immunohistochemical studies have shown its use in its diagnosis. DOG1 antibodies are more sensitive than KIT antibodies in detecting tumors of gastric origin, tumors with epithelioid morphology, and tumors harboring PDGFRA mutation. DOG1 immunoreactivity is rarely observed in other mesenchymal and nonmesenchymal tumor types. These results support the use of DOG1 as a biomarker for GIST.