Anti-DOG1 Antibody

Mouse Monoclonal Antibody specific to DOG1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Discovered on gastrointestinal stromal tumors protein 1, Oral cancer overexpressed protein 2, Transmembrane protein 16A, Tumor-amplified and overexpressed sequence 2

Gene Name

ANO1

NCBI Gene ID

UniProt

Q5XXA6

Cellular Locus

Cell membrane, Cytoplasm

Host

Mouse

Reactivity

Human

Immunogen

Synthetic peptide corresponding to aa 904-986 of human DOG1 (accession no. Q5XXA6).

Target Antigen

Anoctamin-1

Target

DOG1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

IHC

Field of Research

Cancer research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. {PubMed:20056604, PubMed:21984732, PubMed:22178883, PubMed:22946059}.

Additionnal Information

<br><br>Immunohistochemistry: Use at 1- 10ug/ml on paraffin-embedded samples. Antigen retrieval solution: 10mM citrate, pH 6 (boil slides by microwaving in antigen retrieval solution for 12 minutes).User should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human DOG1.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

DOG1 (Discovered on GIST1), also known as TMEM16A and ANO1, has emerged in recent years as a promising biomarker for gastrointestinal stromal tumors (GIST). It was originally discovered through microarray expression profiling analysis as a gene that is highly expressed in GIST, and subsequent immunohistochemical studies have shown its use in its diagnosis. DOG1 antibodies are more sensitive than KIT antibodies in detecting tumors of gastric origin, tumors with epithelioid morphology, and tumors harboring PDGFRA mutation. DOG1 immunoreactivity is rarely observed in other mesenchymal and nonmesenchymal tumor types. These results support the use of DOG1 as a biomarker for GIST.