Anti-WWOX Antibody

Rabbit Polyclonal Antibody specific to WWOX Antibody

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 1.1.1.-, Fragile site FRA16D oxidoreductase, Short chain dehydrogenase/reductase family 41C member 1

Gene Name

WWOX

NCBI Gene ID

UniProt

Q9NZC7

Cellular Locus

Cytoplasm, Nucleus, Mitochondrion, Golgi apparatus

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to amino acids 89-107 (RLAFTVDDNPTKPTTRQRY) of human WWOX conjugated to KLH at the C- terminus.

Target Antigen

WW domain-containing oxidoreductase

Target

WWOX

Clonality

Polyclonal

Type

Antibody

Applications

WB, ELISA

Field of Research

Cancer research

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm. {PubMed:11719429, PubMed:15070730, PubMed:15548692, PubMed:16061658, PubMed:16219768, PubMed:19366691, PubMed:19465938}.

Additionnal Information

Immunoblotting : use at 1:5,000 dilution. A band of approximately 46 kDa is detected. <br><br>Positive control: MCF-7 human breast cancer cell lysate or murine fibroblast L929 cell lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human and mouse WWOX. Sequence identity to chimpanzee and orangutan WWOX is 100%.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

WW domain-containing oxidoreductase, WWOX, also known as WOX1 or FOR, is a proapoptotic protein expressed by a tumor suppressor gene. Because mutation or deletion in the coding region of WWOX is rarely found, it is speculated that the appearance of aberrant transcripts affects progression of many cancers. In addition, protein levels of WWOX, its Tyr33- phosphorylated form, and WOX2 are significantly down-regulated in the neurons of Alzheimer's hippocampi.