Anti-SIRP α Antibody

Rabbit Polyclonal Antibody specific to SIRP α (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Bit, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein α-1, Sirp-α-1, Signal-regulatory protein α-2, Sirp-α-2, Signal-regulatory protein α-3, Sirp-α-3, p84, CD antigen CD172a

Gene Name

SIRPA

Gene ID

140885

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=SIRPA">SIRPA</a>

UniProt

P78324

Accession Number

NP_542970

Cellular Locus

Membrane; Single-pass type I membrane protein.

Host

Rabbit

Reactivity

Human, Mouse, Rat, Bovine

Immunogen

Synthetic peptide corresponding to 17 amino acids within the last 50 amino acids at the C-terminus of human SIRPa.

Target Antigen

Tyrosine-protein phosphatase non-receptor type substrate 1

Target

SIRP α

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC

Field of Research

Neuroscience

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. {PubMed:10469599, PubMed:11509594}.

Additionnal Information

Immunoblotting: use at 0.5-2ug/mL. A band of 75-110kDa is detected. Immunocytochemistry: use at 1-5ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable at 4°C for three months and for at least one year at -20ËšC. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human SIRP alpha 1, 2, and 3.

Formulation

PBS, 0.02% sodium azide.

Target Background

Protein tyrosine phosphatases (PTPases), such as SHP-1 and SHP-2, are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation, or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was cloned from human, rat, mouse, and cattle by several laboratories and was designated SIRPalpha, SHPS-1, and MyD-1. SIRPalpha/SHPS-1 is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR, and src, and is a specific docking protein for SHP-2. SIRPalpha/SHPS-1 has regulatory effects on cellular responses and plays a general role in different physiological and pathological processes.