Human NSE Protein, His Tag (MALS verified)
Human NSE, His Tag (NSE-H5144) is expressed from E. coli cells. It contains AA Met 1 - Leu 434 (Accession # P09104-1) .
Product Specifications
Background
Neuron-specific enolase (NSE) is known to be a cell specific isoenzyme of the glycolytic enzyme enolase. It is also known as gamma-enolase or nolase 2 (ENO2) . It has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. NSE is a highly specific marker for neurons and peripheral neuroendocrine cells. In clinical, NSE could be used as a biomarker for neuronal injury.
Specifications
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 49.3 kDa. The protein migrates as 46-50 kDa under reducing (R) condition (SDS-PAGE) .
Accession Number
P09104-1
Expression Region
Met 1 - Leu 434
Host
E. coli
Target
NSE
Conjugation
Unconjugated
Tag
N-10xHis
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Bioactivity
Immobilized Human NSE, His Tag (Cat. No. NSE-H5144) at 1 μg/mL (100 μL/well) can bind Anti-NSE antibody with a linear range of 1-63 ng/mL (QC tested).
Format
Powder
Buffer
20 mM Tris, pH7.5
Additives
Trehalose
Molecular Weight
49.3 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
500ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/NSE-H5144.pdf
Species
Human
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