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Human NSE Protein, His Tag (MALS verified)

Human NSE, His Tag (NSE-H5144) is expressed from E. coli cells. It contains AA Met 1 - Leu 434 (Accession # P09104-1) .

Product Specifications

Background

Neuron-specific enolase (NSE) is known to be a cell specific isoenzyme of the glycolytic enzyme enolase. It is also known as gamma-enolase or nolase 2 (ENO2) . It has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. NSE is a highly specific marker for neurons and peripheral neuroendocrine cells. In clinical, NSE could be used as a biomarker for neuronal injury.

Specifications

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 49.3 kDa. The protein migrates as 46-50 kDa under reducing (R) condition (SDS-PAGE) .

Accession Number

P09104-1

Expression Region

Met 1 - Leu 434

Host

E. coli

Target

NSE

Conjugation

Unconjugated

Tag

N-10xHis

Source

Human

Stability

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human NSE, His Tag (Cat. No. NSE-H5144) at 1 μg/mL (100 μL/well) can bind Anti-NSE antibody with a linear range of 1-63 ng/mL (QC tested).

Format

Powder

Buffer

20 mM Tris, pH7.5

Additives

Trehalose

Molecular Weight

49.3 kDa

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

500ug*1

Product Datasheet

Https://www.acrobiosystems.com/static/main/products/pdf/ds/NSE-H5144.pdf

Species

Human

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