Anti-EAAC1 Antibody

Mouse Monoclonal Antibody specific to EAAC1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Excitatory amino-acid carrier 1, Neuronal and epithelial glutamate transporter, Sodium-dependent glutamate/aspartate transporter 3, Solute carrier family 1 member 1

Gene Name

SLC1A1

NCBI Gene ID

UniProt

P43005

Cellular Locus

Cell membrane, Apical cell membrane, Cell junction, synapse, synaptosome, Early endosome membrane, Late endosome membrane, Recycling endosome membrane

Host

Mouse

Reactivity

Human

Immunogen

Fusion protein corresponding to aa 1-524 (full-length) of human EAAC1 (accession no. NP_004161.4).

Target Antigen

Excitatory amino acid transporter 3

Target

EAAC1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IP, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7914198, PubMed:7521911, PubMed:8857541, PubMed:26690923, PubMed:21123949). Can also transport L-cysteine (PubMed:21123949). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed:7521911, PubMed:8857541, PubMed:26690923). Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed:8857541, PubMed:26690923). Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli (PubMed:21123949). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity). Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport (By similarity). Negatively regulated by ARL6IP5 (By similarity). {UniProtKB:P51906, UniProtKB:P51907, PubMed:21123949, PubMed:26690923, PubMed:7521911, PubMed:7914198, PubMed:8857541}.

Additionnal Information

Immunoblotting: use at 1-5ug/mL. A band of ~60kDa is detected. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: rat brain membranes.

Storage Conditions

This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Specificity

This antibody recognizes human EAAC1.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

Excitatory amino-acid carrier 1 (EAAC1) is a high-affinity Na+-dependent L- glutamate/D,L-aspartate cell membrane transport protein. It is expressed in brain as well as in several non-nervous system tissues. In brain, EAAC1 is the primary neuronal glutamate transporter. It has a polarized distribution in cells and functions mainly perisynaptically to transport glutamate from the extracellular environment. In the kidney it is involved in renal acidic amino acid reabsorption and amino acid metabolism. EAAC1- associated protein GTRAP3-18 may be important in regulating the metabolic function of EAAC1.