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Tryptophan (Trp) Fluorometric Assay Kit

Product Specifications

Target

Trp

Type

Fluorometric method

Field of Research

Amino Acids and Proteins

Assay Principle

Tryptophan (Trp) is an essential amino acid involved in a variety of biosynthetic processes. It is not only involved in the synthesis of a variety of proteins, but also the precursor of many bioactive substances. The enzyme catalyzes Trp in a series of reactions to produce hydrogen peroxide, which forms a red complex with the fluorescent probe. The fluorescence value of the sample at the excitation wavelength of 535 nm and the emission wavelength of 587 nm can be measured to calculate the content of Trp in the sample.

Assay Performance Time

50 min

Sample Type

Serum; plasma; tissue; cell

Detection Range

0.33-200.00 μmol/L

Sensitivity

0.33 μmol/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

2.5-5.6%

Intra CV

3.4-4.7%

Detection Instrument

Fluorescence Microplate Reader

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