Mouse anti Human p21 WAF-1

Product Specifications

Background

The p21waf1 tumor suppressor protein shows no cross reactivity with the closely related inhibitors .p21waf1 is a specific inhibitor of cdkÕs, and is a tumor suppressor involved in the pathogenesis of a number of malignancies including colon and breast carcinoma

CAS Number

9007-83-4

Synonyms

p21waf1, hp21, Cyclin-dependent kinase inhibitor 1, CDK-interacting Protein 1, Melanoma differentiation-associated Protein 6

UniProt

P38936 (Human)

Host

Mouse

Species Reactivity

Human

Isotype

IgG2a

Clone

DCS-60

Conjugation

Unconjugated

Type

Monoclonal Antibody

Source

Mice were immunized with a recombinant full length p21 protein.

Applications

WB, IP, IHC

Field of Research

Cell Cycle

Purification Method

Protein A/G Chromatography

Assay Principle

1-5 µg /mL for Western Blot, Immunoflurescence, Immunoprecipitation (co-precipitates cdk4/cdk6)

Stability

See expiration date on vial

Concentration

See vial for Concentration

Form

Provided as solution in phosphate buffered saline with 0,08% sodium azide

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

References & Citations

1) R. Erber, et.al. (1997) Pred. Oncol. 74, 383-389 _x000D_ 2. Shen, J., et al. ‘Identification and Validation of Differences in Protein Levels in Normal, Premalignant, and Malignant Lung Cells and Tissues Using High-Throughput Western Array and Immunohistochemistry.’ Cancer Res., 66, 11194-11206 (2006).