Sheep anti Dog IgG (Fc specific)

The reactivity of the antiserum is restricted to the Fc part of the IgG molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal dog serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No precipitation reaction is obtained with purified IgA, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in dog serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of dog IgG from serum or plasma. The antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive techniques. For such uses labelled and unlabelled cytochemical grade reagents raised in goats meeting the necessary additional specificity and performance requirements are available.

Highly purified normal IgG isolated from pooled dog serum was used for immunization of the sheep. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Precipitation assays. In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.

Delipidated, heat inactivated, lyophilized, stable whole antiserum. 0,01% sodium azide; Total protein and IgG concentrations in the antiserum are comparable to those of pooled; Normal sheep serum; No foreign proteins added; Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C; Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance of the antiserum Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day