Biotinylated Human Claudin-6 Full Length Protein, His, Avitag™&Flag Tag (Detergent)

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. Claudin-6, also knows as CLDN6, is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. And it is one of the entry cofactors for hepatitis C virus. The methylation of CLDN6 may be involved in esophageal tumorigenesis. The gene of CLDN6 is adjacent to another family member CLDN9 on chromosome 16.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the N-terminus and a Flag tag at the C-terminus followed by an Avi tag (Avitag™) . The protein has a calculated MW of 28.6 kDa. The protein migrates as 28-30 kDa, 50-55 kDa and 65-180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P56747

Expression Region

Ala 2 - Val 220

Host

Insect Cells

Target

Claudin-6

Conjugation

Biotin-labeled

Tag

N-10xHis & C-Flag & C-Avi

Source

Human

Stability

The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

85%

Bioactivity

Immobilized Biotinylated Human Claudin-6 Protein, His,Avitag&Flag Tag (Cat. No. CL6-H85D3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-Claudin-6 Antibody, Human IgG1 with a linear range of 0.05-2 ng/mL (QC tested).