Anti-Piccolo Antibody

Mouse Monoclonal Antibody specific to Piccolo

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Aczonin, Brain-derived HLMN protein, Multidomain presynaptic cytomatrix protein

Gene Name

Pclo

NCBI Gene ID

UniProt

Q9QYX7

Cellular Locus

Cell junction, synapse, presynaptic active zone

Host

Mouse

Reactivity

Human, Rat

Immunogen

Full-length human Piccolo protein (accession no. BC001304.1)

Target Antigen

Protein piccolo

Target

Piccolo

Clonality

Monoclonal

Isotype

IgG2b

Type

Antibody

Applications

WB, ELISA

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed:19812333). After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (By similarity). At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis (By similarity). Organizes as well the readily releasable pool of synaptic vesicles and safeguards a fraction of them to be not immediately available for action potential-induced release (By similarity). Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy (PubMed:28231469) (By similarity). Mediates also synapse to nucleus communication leading to rUniProtKB:Q9JKS6, PubMed:19812333, PubMed:28231469}.

Additionnal Information

Immunoblotting: use at 1-10ug/ml. A band of ~550kDa is detected ELISA: use at 1-10ug/ml with Piccolo on the solid phase. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human and rat Piccolo isoforms (other species not yet tested). It does not cross-react with Bassoon.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Piccolo is a novel component of the presynaptic cytoskeletal matrix (PCM) assembled at the active zone of neurotransmitter release. Piccolo is a multidomain zinc finger protein structurally related to Bassoon, another PCM protein. Both proteins share components of glutamatergic and GABAergic CNS synapses. Piccolo zinc fingers interact with the dual prenylated rab3A and VAMP2/Synaptobrevin II receptor PRA1. Piccolo is involved in endo- and exocytosis of synaptic vesicles and has been shown to interfere with clathrin- mediated endocytosis by binding to the F- actin and dynamin bind protein Abp1.