Mouse ST2 ELISA Kit
Product Specifications
Abbreviation
ST2
UniProt
P14719
Cross Reactivity
This kit recognizes Mouse ST2 in samples. No significant cross-reactivity or interference between Mouse ST2 and analogues was observed
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Cancer; Cell Biology; Cardiovascular; Immunology; Signal transduction
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ST2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ST2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ST2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ST2. You can calculate the concentration of Mouse ST2 in the samples by comparing the OD of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.31-20 ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
0.19 ng/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,12 months
Product Datasheet
https://789.bio/ea/ayehth
Product MSDS
https://789.bio/eb/xmPGCC
Species
Mouse
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Mouse sT2 concentrations in serum, plasma and other biological fluids.
Available Sizes
Curated Selection
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