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Cytokeratin 8 KRT8 Mouse Monoclonal Antibody

Anti-Cytokeratin 8 KRT8 Antibody (monoclonal, B5I1) . Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Product Name Alternative

CARD2; Cell and organelle markers; CK 8; CK8; CYK8; Cytokeratin 8; Cytoskeleton Marker; K2C8; K8; keratin 8; KO; KRT8; Type II keratin Kb8

UniProt

P05787

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Cytokeratin 8 recombinant protein (Position: D107-K325) . Human Cytokeratin 8 shares 95.4% and 94.5% amino acid (aa) sequence identity with mouse and rat Cytokeratin 8, respectively.

Target

Keratin, type II cytoskeletal 8

Clonality

Monoclonal

Clone

B5I1

Conjugation

Unconjugated

Field of Research

Cell Biology, Immunology & Inflammation, Protein Biochemistry, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Dilution

Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunofluorescence, 2μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry (Fixed), 1-3μg/1x10^6 cells

Form

Lyophilized

Molecular Weight

54 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Tested Applications

FC, ICC, IF, IHC, IHC-Fr, WB

Host or Source

Mouse

Preservative

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Isotype

Mouse IgG2b

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