Maleimide Activated Alkaline Phosphatase

Maleimide Activated Alkaline Phosphatase is commonly used to facilitate the conjugation of alkaline phosphatase to proteins, peptides and ligands containing sulfhydryl (-SH) groups for using it as an enzyme-linked detection reagent. Maleimide Activated Alkaline Phosphatase consists of alkaline phosphatase that has been modified by hetero bi-functional cross linker Sulfo-SMCC and contains several maleimide groups per alkaline phosphatase molecule while maintaining most of the enzyme activity. After conjugation, the activated alkaline phosphatase will form a covalent interaction with the peptides or ligands containing sulfhydryl groups and can be used as a probe for detection in various assays such as ELISA, western blot etc.