Human ROBO1 Protein, His Tag (MALS verified)

ROBO1 is a member of the ROBO immunoglobulin superfamily of proteins, and it plays a crucial role in cell motility and migration during embryogenesis and organogenesis. In addition, evidence showed that ROBO1 might drive migration and invasion in malignant cells, such as glioma and breast cancer, which might play a role in cancer aggressiveness. In contrast, some studies suggested that ROBO1 pathways play a key role in tumors by acting as a tumor suppressor, especially in cell invasion.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 97.5 kDa. The protein migrates as 100-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q9Y6N7-1

Expression Region

GIn 26 - Pro 897

Host

HEK293

Target

ROBO1

Conjugation

Unconjugated

Tag

C-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.2 EU per μg

Purity

95%

Bioactivity

Immobilized Anti-ROBO1 Antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Human ROBO1, His Tag (Cat. No. RB1-H52H3) with a linear range of 8-125 ng/mL (QC tested).