Bat coronavirus Cp/Yunnan2011 Spike Trimer Protein (R653A, KV954-955PP), His Tag (MALS verified)
Bat coronavirus Cp/Yunnan2011 Spike Trimer, His Tag (SPN-B52H5) is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. It contains AA Ala 13 - Pro 1181 (Accession # R9QTH3 (R653A, KV954-955PP) ) .
Product Specifications
Background
The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 134.9 kDa. The protein migrates as 160-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
R9QTH3 (R653A, KV954-955PP)
Expression Region
Ala 13 - Pro 1181
Host
HEK293
Target
Spike protein
Conjugation
Unconjugated
Tag
C-10xHis
Source
Bat coronavirus
Stability
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Format
Powder
Buffer
0.1 M Sodium citrate, pH5.5
Additives
Trehalose
Molecular Weight
134.9 kDa
Additionnal Information
Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
50ug*1
Host or Source
HEK293
Species
Bat coronavirus
Protein ID
R9QTH3 (R653A, KV954-955PP)
Preservative
Trehalose
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