Anti-c-myc Antibody

Goat Polyclonal Antibody specific to c-myc

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Class E basic helix-loop-helix protein 39, bHLHe39, Proto-oncogene c-Myc, Transcription factor p64

Gene Name

MYC

NCBI Gene ID

UniProt

P01106

Cellular Locus

Nucleus, nucleoplasm, Nucleus, nucleolus

Host

Goat

Reactivity

C-Myc

Immunogen

Amino acids 410-419 (EQKLISEEDL) of human myc conjugated to KLH.

Target Antigen

Myc proto-oncogene protein

Target

c-myc

Clonality

Polyclonal

Type

Antibody

Applications

WB, IP, ICC, ELISA

Field of Research

Epitope Tags

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Transcription factor that binds DNA in a non-specific manner, yet also specifically rUniProtKB:P01108, PubMed:20010808, PubMed:24940000, PubMed:25956029}.

Additionnal Information

Immunoblotting: use at a dilution of 1:1,000-1:20,000.Detection of c-myc-tagged protein in 200, 100, and 50ng of E. coli lysate containing tagged fusion protein with #18824 diluted 1:25,000. Immunocytochemistry: use at a dilution of 1:100-1:400. ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100- 1:500. Immunoprecipitation: use at 1-4ug/mg lysate These are recommended dilutions. Endusers should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at 2-8°C.

Specificity

Antibody was affinity-purified on the peptide.

Formulation

PBS, pH 7.2, 0.09% sodium azide.

Buffer pH

pH 7.2

Target Background

c-myc is an epitope tag often constructed on the N- or C-terminus of a protein to allow for detection and analysis of the protein. The peptide sequence of the myc tag is EQKLISEEDL (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu). The c-myc tag is approximately 1202 Daltons in atomic mass and includes 10 amino acids.