SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), His Tag (MALS verified)

SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), His Tag (SPD-C52Hr) is expressed from human 293 cells (HEK293) . It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (K417T, E484K, N501Y) ) . K417T/ E484K/ N501Y mutations were identified in the SARS-CoV-2 Gamma variant (Pango lineage: P.1; other names: 20J/501Y.V3) .

Product Specifications

Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.5 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

QHD43416.1 (K417T, E484K, N501Y)

Expression Region

Arg 319 - Lys 537

Host

HEK293

Target

Spike RBD

Conjugation

Unconjugated

Tag

C-10xHis

Source

SARS-CoV-2

Stability

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized SARS-CoV-2 S protein RBD (K417T, E484K, N501Y), His Tag (Cat. No. SPD-C52Hr) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.8-3 ng/mL (QC tested).