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Hsp27/HSPB1 Rabbit Polyclonal Antibody

Hsp27/HSPB1 Rabbit Polyclonal Antibody

Product Specifications

Product Name Alternative

Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27, HSP28

UniProt

P04792

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Hsp27 recombinant protein (Position: M1-K205) . Human Hsp27 shares 83% amino acid (aa) sequence identity with mouse Hsp27.

Target

Heat shock protein beta-1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology, Cardiovascular Research, Cell Biology, Neuroscience, Protein Biochemistry, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Dilution

Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, - Immunocytochemistry/Immunofluorescence, 2μg/ml, Human, - Immunoprecipitation, 0.5-2 μg/ml, Human Flow Cytometry (Fixed), 1-3μg/mlx10^6 cells, Human

Form

Lyophilized

Molecular Weight

27 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Tested Applications

FC, ICC, IF, IHC, IHC-Fr, IP, WB

Host or Source

Rabbit

Preservative

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Isotype

Rabbit IgG

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