Anti-Alginate, Pseudomonas-reactive, Antibody

Mouse Monoclonal Antibody specific to Alginate

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Alginate Mouse Monoclonal Antibody

Host

Mouse

Reactivity

Pseudomonas

Immunogen

Sodium alginate conjugated to KLH

Target

Alginate, Pseudomonas-reactive,

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

ELISA

Field of Research

Infectious Disease

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

ELISA: Use at 0.5-20ug/ml with alginate on the solid phase. Working concentrations for these antibodies are dependent on the purity and concentration of alginate in samples tested.

Storage Conditions

These antibodies are stable for at least one (1) year at -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.

Specificity

Alginate

Formulation

PBS, pH 7.4, 50% glycerol, 0.1% sodium azide.

Buffer pH

pH 7.4

Target Background

Pulmonary infection by mucoid, alginate-producing Pseudomonas aeruginosa is the leading cause of mortality among patients suffering from cystic fibrosis. During early childhood, cystic fibrosis patients are colonized by multiple bacterial pathogens including nonmucoid P. aeruginosa. The appearance of mucoid isolates indicates progression to chronic infections. Recent evidence indicates that P. aeruginosa is most resistant to antibiotics when the infecting cells are present as a biofilm, as they appear to be in the lungs of cystic fibrosis patients.