Anti-Alginate, Pseudomonas-reactive, Antibody

ELISA: Use at 0.5-20ug/ml with alginate on the solid phase. Working concentrations for these antibodies are dependent on the purity and concentration of alginate in samples tested.

Pulmonary infection by mucoid, alginate-producing Pseudomonas aeruginosa is the leading cause of mortality among patients suffering from cystic fibrosis. During early childhood, cystic fibrosis patients are colonized by multiple bacterial pathogens including nonmucoid P. aeruginosa. The appearance of mucoid isolates indicates progression to chronic infections. Recent evidence indicates that P. aeruginosa is most resistant to antibiotics when the infecting cells are present as a biofilm, as they appear to be in the lungs of cystic fibrosis patients.