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Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Mouse)

Antibody coupling magnetic beads, suitable for IP/ CO-IP experiment, protein purification.

Product Specifications

Background

Immunoprecipitation (Immunoprecipitation, IP) is a small affinity purification method using specific antibodies fixed on solid-phase supports such as magnetic beads or agarose. As an important part of many proteomics-related studies, IP can be used to detect the existence of proteins, relative abundance, up-and-down expression of proteins, stability and interaction of proteins and so on.

CAS Number

9007-83-4

Tag

Universal IP/Co-IP Toolkit (Magnetic Beads/Anti-Mouse)

Type

Sample Preparation and detection

Components

• Non-Denaturing Lysis Buffer-25 mL• 10XWash Buffer-20 mL• Protein A/G Magnetic Beads-0.5 mL• Elution Buffer-2 mL• Neutralization Buffer-0.2 mL• 100XProteinase Inhibitor Cocktail-0.2 mL• Mouse IgG (1 mg/mL) -30 μL• IPKine™ HRP, Goat Anti-Mouse IgG LCS-30 μL

Precautions

The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes.

Features & Benefits

• Efficient: Efficient antibody binding capacity and low protein non-specific adsorption rate, saving antibody consumption.• Convenient and universal: Contain all the necessary buffers needed for IP/Co-IP and WB experiments, it can meet the requirements of sample IP/Co-IP or WB at the same time. • Reliable and stable: It contains ready-to-use IP negative control, which can eliminate the non-specific binding of IgG itself to the target protein or other specific biomolecules to ensure the specificity of IP antibodies, and contains unique IPkine™ secondary antibodies to perfectly eliminate heavy chain interference.

Shipping Conditions

Blue ice

Storage Conditions

Store according to the recommended storage conditions of each component.

Applications Notes

Based on the pain points of the traditional IP/Co-IP experiment, Abbkine developed a Universal IP/Co-IP Toolkit (Magnetic Beads) ; After designed and researched, the toolkit contains: optimized natural and denatured lysate, protein A/G magnetic beads, IP negative control, IPkineTM second antibody, it can meet the IP/Co-IP needs of most users

Other Product Names

Immunoprecipitation; IP; Co-IP; protein A; protein G; protein A/G; IP kit; CO-IP kit; IP toolkit; CO-IP toolkit; Immunoprecipitation kit; CO-Immunoprecipitation kit

Tested Applications

IP, Co-IP, WB

Frequently Asked Questions

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