Anti-TrpV3 Cation Channel Antibody

Mouse Monoclonal Antibody specific to TrpV3 Cation Channel

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-TrpV3 Cation Channel Mouse Monoclonal Antibody

UniProt

Q4QYD9

Accession Number

NP_001020928

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to aa 458-474 (cytoplasmic C-terminus) of rat TrpV3 (accession no. NP_001020928).

Target Antigen

Heat sensitive channel TRPV3

Target

TrpV3 Cation Channel

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, IHC, ICC/IF, IP, AM

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at 1-10ug/mL. A band of ~70kDa is detected.Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/mL Immunofluorescence: use at 1-10ug/mL These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: COS cell lysate transiently transfected with TrpM7.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat TrpV3.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. The TrpV3 protein belongs to a family of nonselective cation channels that function in a variety of processes including temperature sensation and vasoregulation. The thermosensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin and are activated at distinct physiological temperatures. The TrpV3 channel is activated at temperatures between 22 and 40 C. The TrpV3 gene is in close proximity to the TrpV1 gene on chromosome 17, and studies suggest that the corresponding two proteins associate with each other to form heteromeric channels.