Anti-PfHsp60 Antibody

Rabbit Polyclonal Antibody specific to PfHsp60

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-PfHsp60 Rabbit Polyclonal Antibody

UniProt

P34940

Accession Number

XM_001347402.1

Cellular Locus

Mitochondrion matrix

Host

Rabbit

Reactivity

P. Facilparum

Immunogen

Recombinant full-length PfHsp60 (Accession no. XM_001347402.1)

Target Antigen

Chaperonin CPN60, mitochondrial

Target

PfHsp60

Clonality

Polyclonal

Type

Antibody

Applications

WB, ICC/IF, IHC

Field of Research

Heat Shock& Stress Proteins

Purification Method

Purified by Protein A affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

Additionnal Information

Immunoblotting: use at 1ug/mL. A band of ~62kDa is detected. <br><br>These are recommended concentrations. <br><br>Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20��C.

Specificity

This antibody recognizes P. falciparum Hsp60 and cross-reacts with E.coli Hsp60/GroEl.

Formulation

PBS (1.83mg/ml), pH 7.4, 50% glycerol, 0.09% sodium azide

Buffer pH

pH 7.4

Target Background

Hsp60 is an abundant, constitutively produced heat shock protein that increases in cellular concentration in response to cell shock. It is found in multiple species and is notably homologous in bacteria, plants, and mammals. Hsp60 of Plasmodium falciparum is a polypeptide of 577 amino acids with a calculated molecular weight of 62158 Da. The primary structure of P. falciparum hsp60 contains a putative mitochondrial targeting peptide at its amino- terminus and a GGM motif at its carboxyl- terminus. The overall structure exhibits strong conservation (approximately 50%) to the hsp60 from human and other eukaryotes, but only low homology (< 30%) to a recently reported P. falciparum chaperonin 60 gene. The P. falciparum hsp60 gene is located on chromosome 10. During heat shock, the level of hsp60 transcript in blood stage parasites increases significantly and its accumulation correlates with the duration of the induction.