Anti-ARC (CT) Antibody

Rabbit Polyclonal Antibody specific to ARC (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Apoptosis repressor with CARD, Muscle-enriched cytoplasmic protein, Myp, Nucleolar protein of 30 kDa, Nop30

Gene Name

NOL3

Gene ID

8996

NCBI Gene ID

UniProt

O60936

Accession Number

NP_003937

Cellular Locus

[Isoform 1]: Nucleus, nucleolus

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 191- 208 of human ARC.

Target Antigen

Nucleolar protein 3

Target

ARC (CT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IHC-P, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

[Isoform 1]: May be involved in RNA splicing. {PubMed:10196175}.; [Isoform 2]: Functions as an apoptosis repressor that blocks multiple modes of cell death. Inhibits extrinsic apoptotic pathways through two different ways. Firstly by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly (By similarity). SUniProtKB:Q62881, UniProtKB:Q9D1X0, PubMed:15004034, PubMed:15509781}.

Additionnal Information

Immunoblotting: use at 1-10ug/mL. <br><br>Immunohistochemistry: use at 5ug/mL.Immunohistochemistry of ARC in human heart tissue <br><br>Positive control: Whole cell lysate of HeLa, KB, and A549 cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes full-length ARC (25 kD).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

A novel CARD domain-containing protein was recently identified and designated ARC (apoptosis repressor with CARD). ARC interacts with caspase-2 and -8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1, and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue.