Anti-ECS Antibody, HRP conjugate

Goat Polyclonal Antibody specific to ECS HRP

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-ECS HRP Goat Polyclonal Antibody

Gene ID

5651

UniProt

P98073

Cellular Locus

Membrane, Single-pass type II membrane protein

Host

Goat

Reactivity

Ecs

Immunogen

DDDDK conjugated to KLH.

Target

ECS

Clonality

Polyclonal

Isotype

Whole IgG

Type

Antibody

Applications

WB, IHC, ICC, ELISA

Field of Research

Epitope Tags

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.

Additionnal Information

Immunoblotting: use at a dilution of 1:1,000-1:30,000. Immunocytochemistry: use at a dilution of 1:200-1:500 ELISA: use at a dilution of 1:10,000-1:100,000. These are recommended dilutions. End users should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at 2-8°C.

Specificity

Antibody was affinity-purified on the peptide.

Formulation

Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400

Target Background

Enterokinase, also known as enteropeptidase, is a serine protease that catalyzes the hydrolysis of peptide bonds in proteins but, unlike other kinases, does not catalyze transfer of phosphate groups. Enterokinase cleaves proteins following a lysine at an enterokinase cleavage site, or ECS (Asp-Asp-Asp-Asp-Lys). In vivo, this cleavage results in trypsin-dependent activation of other pancreatic zymogens, such as chymotrypsinogen, proelastase, procarboxypeptidase and prolipase in the lumen of the gut.