Mouse anti Human CD44

CD44 is a 90-kD (GP90), collagen-binding, membrane glycoprotein, termed extracellular matrix receptor III (ECMR III), that is homologous to the lymphocyte homing receptor and CD44 antigen. CD44 is abundantly expressed in many epithelial tissues, and is localized predominantly to filopodia in cultured keratinocytes. CD44 is a polymorphic family of related membrane proteoglycans and glycoproteins possessing extensive diversity in both glycosylation and core protein sequence. Human neonatal foreskin keratinocytes (HFKs) and QG56 lung squamous carcinoma cells express an alternatively spliced form of the CD44 core protein (termed CD44E) that contains an additional 132 amino acids in the carbohydrate attachment region of the extracellular domain. HFKs, HT1080 fibrosarcoma and QG56 cells, as well as many other Human cells, contain varying ratios of GP90. Differences in mass are due primarily to variation in carbohydrate moieties, including sulfated asparginelinked glycopeptides (GP), chondroitin sulfate (CS), and heparan sulfate (HS) glycosaminoglycans, as well as O-linked mucin and polylactosamine structure(s).

PBMC: Add10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20ºC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

1. Shedding of the CD44 adhesion molecule from leukocytes induced by anti-CD44 monoclonal antibody simulating the effect of a natural receptor ligand. Bazil V; Horejsi V J Immunol 1992 Aug 1;149(3):747-53 2. Requirements for hyaluronic acid binding by CD44: a role for the cytoplasmic domain and activation by antibody. Lesley J; He Q; Miyake K; Hamann A; Hyman R; Kincade PW J Exp Med 1992 Jan 1;175(1):257-66 3. Variations in the cytoskeletal interaction and posttranslational modification of the CD44 homing receptor in macrophages. Camp RL; Kraus TA; Pure E Cell Biol 1991 Dec;115(5):1283-92 4. Distinct effects of two CD44 isoforms on tumor growth in vivo. Sy MS; Guo YJ; Stamenkovic I J Exp Med 1991 Oct 1;174(4):859-66 5. Participation in normal immune responses of a metastasis-inducing splice variant of CD44 [see comments] Arch R; Wirth K; Hofmann M; Ponta H; Matzku S; Herrlich P; Zoller Comment in: Science 1992 Jul 31;257(5070):614 Science 1992 Jul 31;257(5070):682-5 6. Cytokine production by mature and immature CD4-CD8- T cells. Alpha beta-T cell receptor+ CD4-CD8- T cells produce IL-4. Zlotnik A; Godfrey DI; Fischer M; Suda T J Immunol 1992 Aug 15;149(4):1211-5 7. Inhibition of tumor growth in vivo with a soluble CD44-immunoglobulin fusion protein. Sy MS; Guo YJ; Stamenkovic I J Exp Med 1992 Aug 1;176(2):623-7