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Anti-FAK (Phospho-Tyr861) Antibody

Rabbit Polyclonal Antibody specific to FAK (Phospho-Tyr861)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-FAK (Phospho-Tyr861) Rabbit Polyclonal Antibody

UniProt

Q05397

Host

Rabbit

Reactivity

Human

Immunogen

Peptide sequence that includes phosphorylation site of tyrosine 861 (H-I-Y(p)-Q-P) derived from human FAK.

Target

FAK (Phospho-Tyr861)

Clonality

Polyclonal

Type

Antibody

Applications

WB

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~125kDa is detected. <br><br>These are recommended working dilutions. <br><br>End user should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human FAK only when phosphorylated at tyrosine 861.

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

Focal Adhesion Kinase, FAK (also known as PTK2 protein tyrosine kinase 2, PTK2) is a protein that, in humans, is encoded by the PTK2 gene. FAK is a focal adhesion-associated protein kinase involved in cellular adhesion and spreading processes. It has been shown that when FAK is blocked, breast cancer cells become less metastatic due to decreased mobility.

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