4-Nitrophenyl phosphate

4-Nitrophenyl phosphate (p-Nitrophenyl phosphate) is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases.4-Nitrophenyl phosphate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1].

Product Specifications

CAS Number

[330-13-2]

Product Name Alternative

P-Nitrophenyl phosphate

UNSPSC

12352200

Hazard Statement

H302, H315, H319, H335

Target

Biochemical Assay Reagents

Type

Reference compound

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/4-nitrophenyl-phosphate.html

Purity

99.48

Solubility

DMSO : 100 mg/mL (ultrasonic) |H2O : 100 mg/mL (ultrasonic)

Smiles

O=P(O)(O)OC(C=C1)=CC=C1[N+]([O-])=O

Molecular Formula

C6H6NO6P

Molecular Weight

219.09

Precautions

H302, H315, H319, H335

References & Citations

[1]Lountos GT, et al. Structural analysis of human dual-specificity phosphatase 22 complexed with a phosphotyrosine-like substrate. Acta Crystallogr F Struct Biol Commun. 2015 Feb;71 (Pt 2) :199-205. |[2]Qi S , et al. Development of a facile and sensitive method for detecting alkaline phosphatase activity in serum with fluorescent gold nanoclusters based on the inner filter effect. Analyst. 2020 Jun 7;145 (11) :3871-3877.