Biotinylated Human HLA-A*02:01&B2M&RHAMM-R3 (ILSLELMKL) Complex Protein (Monomer, MALS verified)

Biotinylated Human HLA-A*02:01&B2M&RHAMM-R3 (ILSLELMKL) Complex Protein (HLR-H82E6) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & ILSLELMKL peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & ILSLELMKL) .

Product Specifications

Background

The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with a higher risk of breast cancer. Alternatively, spliced transcript variants encoding different isoforms have been noted for this gene. The Human HLA-A*0201 RHAMM-R3 (ILSLELMKL) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and ILSLELMKL peptide of the RHAMM-R3.

Specifications

Biotinylated Human HLA-A*02:01&B2M&RHAMM-R3 (ILSLELMKL) Complex Protein is produced by co-expression of HLA and B2M loaded with RHAMM-R3 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 40-44 kDa and 13 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & ILSLELMKL

Expression Region

Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & ILSLELMKL peptide

Host

HEK293

Target

HLA-A*0201 & B2M & RHAMM-R3 (ILSLELMKL)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human HLA-A*02:01&B2M&RHAMM-R3 (ILSLELMKL) Complex Protein (Cat. No. HLR-H82E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).