SingleFlowEx CD62L APC

SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

Product Specifications

Certification

RUO (GMP)

Clone

LT-TD180

Applications

FC (QC tested)

Concentration

0.050 mg/mL

Format

APC

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Components

1 vial of monoclonal antibody reagent.The content (1 mL) is sufficient for min. 100 Tests.

References & Citations

*Kuttruff S, Koch S, Kelp A, Pawelec G, Rammensee HG, Steinle A: NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood. 2009 Jan 8;113 (2) :358-69., URL: https://www.ncbi.nlm.nih.gov/pubmed/18922855, *Linnebacher M, Wienck A, Boeck I, Klar E: Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260 (FTO) . J Biomed Biotechnol. 2010;2010:841451., URL: https://www.ncbi.nlm.nih.gov/pubmed/20339516, *Simone R, Zicca A, Saverino D: The frequency of regulatory CD3+CD8+CD28- CD25+ T lymphocytes in human peripheral blood increases with age. J Leukoc Biol. 2008 Dec;84 (6) :1454-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/18780874, *Use of this product has not been published., URL:

Other References

*Ramachandran V, Williams M, Yago T, Schmidtke DW, McEver RP: Dynamic alterations of membrane tethers stabilize leukocyte rolling on P-selectin. Proc Natl Acad Sci U S A. 2004 Sep 14;101 (37) :13519-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/15353601?ordin, *Ding Z, Issekutz TB, Downey GP, Waddell TK: L-selectin stimulation enhances functional expression of surface CXCR4 in lymphocytes: implications for cellular activation during adhesion and migration. Blood. 2003 Jun 1;101 (11) :4245-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/12609846?ordin, *Simon SI, Burns AR, Taylor AD, Gopalan PK, Lynam EB, Sklar LA, Smith CW: L-selectin (CD62L) cross-linking signals neutrophil adhesive functions via the Mac-1 (CD11b/CD18) beta 2-integrin. J Immunol. 1995 Aug 1;155 (3) :1502-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/7543524?ordina, *von Andrian UH, Chambers JD, Berg EL, Michie SA, Brown DA, Karolak D, Ramezani L, Berger EM, Arfors KE, Butcher EC. L-selectin mediates neutrophil rolling in inflamed venules through sialyl LewisX-dependent and -independent recognition pathways. Blood. 1993 Jul 1;82 (1) :182-91., URL: https://www.ncbi.nlm.nih.gov/pubmed/7686786?ordina, *Von Andrian UH, Hansell P, Chambers JD, Berger EM, Torres Filho I, Butcher EC, Arfors KE: L-selectin function is required for beta 2-integrin-mediated neutrophil adhesion at physiological shear rates in vivo. Am J Physiol. 1992 Oct;263 (4 Pt 2) :H1034-44., URL: https://www.ncbi.nlm.nih.gov/pubmed/1384360?ordina