Anti-Replication Factor C (p37) Antibody

Mouse Monoclonal Antibody specific to Replication Factor C p37

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Activator 1 40 kDa subunit, A1 40 kDa subunit, Activator 1 subunit 2, Replication factor C 40 kDa subunit, RF-C 40 kDa subunit, RFC40

Gene Name

RFC2

NCBI Gene ID

UniProt

P35250

Cellular Locus

Nucleus

Host

Mouse

Reactivity

Human, Yeast

Immunogen

Recombinant protein corresponding to the p37 subunit of human Replication Factor C expressed in E. coli.

Target Antigen

Replication factor C subunit 2

Target

Replication Factor C (p37)

Clonality

Monoclonal

Isotype

IgG2b

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Cancer research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP (By similarity). {ECO:0000250}.

Additionnal Information

Immunoblotting: use at 1:2,000 dilution. A band of 37 kD is detected. <br><br>Positive controls: HeLa cell lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes the p37 subunit of human and yeast (S. cerevisiae) Replication Factor C (RFC). RFC is a heteropentameric protein consisting of p140, p40, p38, p37, and p36 subunits, RFC participates in targeting DNA polymerase delta processivity factor (PCNA) on to a primed DNA template during DNA synthesis and repair. RFC has also been associated with DNA repair in conjunction with a PCNA-like heterotrimer of Hus1, Rad1, and Rad9.