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293 [HEK-293] Cell Line

Product Specifications

Synonyms

Hek293;HEK-293;HEK 293;HEK:293;293;293 HEK;Human Embryonic Kidney 293

Cell Type

Transformed cell line

Growth Properties

Adherent

Medium

CM-0001

Media

CM-0001

Shipping Conditions

Dry Ice

Storage Conditions

For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Notes

The cell line does not adhere to the substrate when left at room temperature for any length of time, therefore, live cultures may be received with the cells detached.The cells will re-attach to the flask over a period of several days in culture at 37°C.

Citation 01

https://www.nature.com/articles/s41591-022-02194-3

Citation 02

https://link.springer.com/article/10.1007/s42114-024-01141-9

Citation 03

https://www.sciencedirect.com/science/article/pii/S1748013222002018

Citation 04

https://www.nature.com/articles/s41467-022-30338-z

Citation 05

https://www.nature.com/articles/s41467-017-02355-w

Citation 06

https://www.nature.com/articles/s41467-025-63911-3

Citation 07

https://www.nature.com/articles/s41418-025-01575-8

Citation 08

https://www.nature.com/articles/s41467-025-64069-8

Citation 09

https://www.nature.com/articles/s41467-025-60768-4

Citation 10

https://www.nature.com/articles/s41467-025-56574-7

Citation 11

https://www.sciencedirect.com/science/article/pii/S1748013224004481

Citation 12

https://www.sciencedirect.com/science/article/pii/S1385894724064301

Species

Human

Tissue

Embryo; kidney

Biosafety Level

BSL-2

Incubation Temperature

37℃

Morphology

Epithelial-like

Doubling Time

~24-30 hours

Split Ratio

1:4-1:8

Frequently Asked Questions

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