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PD-L1 antibody

PD-L1 antibody validated for immunohistochemistry on 76 different Normal Tissues

Product Specifications

Product Name Alternative

B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; Programmed cell death 1 ligand 1

UniProt

Q9NZQ7

Reactivity

Human

Immunogen

KLH-conjugated linear peptide corresponding to human PD-L1

Clonality

Monoclonal

Clone

MSVA-711R

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Dilution

1:100-1:200

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Alveolar macrophages, tonsil crypt epithelium and the placental cytotrophoblast show strong, predominantly membranous positivity. Follicle centre macrophages should show at least moderate intensity staining. Negative Control: Appendix (negative staining of all epithelial cells) Cellular Localization: Predominantly membranous Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Tested Applications

IHC

Host or Source

Rabbit

Isotype

IgG

Available Sizes

Frequently Asked Questions

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