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MUC5AC antibody

MUC5AC antibody validated for immunohistochemistry on 76 different Normal Tissues

Product Specifications

Product Name Alternative

Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A and C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin

UniProt

P98088

Reactivity

Human

Immunogen

M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le (a-b) patient

Clonality

Monoclonal

Clone

MSVA-109M

Conjugation

Unconjugated

Dilution

1:100-1:200

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Gastric mucosa: A strong cytoplasmic MUC5AC staining should be seen in the surface epithelium. Negative Control: Kidney: MUC5AC staining should be absent. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Tested Applications

IHC

Host or Source

Mouse

Isotype

IgG1, kappa

Available Sizes

Frequently Asked Questions

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