MUC5AC antibody

MUC5AC antibody validated for immunohistochemistry on 76 different Normal Tissues

Product Specifications

Product Name Alternative

Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A and C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin

UniProt

P98088

Reactivity

Human

Immunogen

M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le (a-b) patient

Clonality

Monoclonal

Clone

MSVA-109M

Conjugation

Unconjugated

Dilution

1:100-1:200

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Gastric mucosa: A strong cytoplasmic MUC5AC staining should be seen in the surface epithelium. Negative Control: Kidney: MUC5AC staining should be absent. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.